Bio-Rad CFX96 (qPCR)

Bio-Rad CFX96 (qPCR)2018-11-14T15:37:58+00:00

Our plug-in will allow you to incorporate your CFX96 into a wide variety of protocols. Or look at some of our pre-configured workcells that support the preparation and/or loading of plates.

Automated qPCR Prep (AqP)

Building your qPCR reactions has never been easier or more affordable. The Hudson Robotics SOLO is the ideal pipettor for building the most complex sample set for your qPCR pipeline. Whether you are building a few qPCR reactions from 1.5mL source tubes or running serial dilutions on thousands of samples per day, there is a SOLO configuration to get the job done. Typical SOLO Configurations can include:

  • SOLO Pipettor in single or multi channel configurations
  • Tube Racks for almost any incoming sample tube
  • Optional Automated qPCR plate delivery
    1. PlateCrane EX
    2. LabLinx
    3. ArmLink

 

Automated qPCR Loading (AqL)

Delivering qPCR plates ready for cycling and detection is a popular application best served by a Hudson Robotics PlateCrane EX. A typical Automated qPCR Loading work cell can include 1 qPCR thermal cycler and up to 8 or more qPCR thermal cyclers. Automated qPCR Loading workcells include:

  • PlateCrane EX microplate robot
  • qPCR Thermal cycler(s)
  • Barcode Reader
  • Input and Output for qPCR Plate Storage
  • Easy to use and Flexible Softlinx Software

Automated qPCR System (AqS)

A fully automated workcell for setting up qPCR reactions, sealing plates, thermal cycling and data collection. qPCR reactions are assembled using the Hudson Robotics SOLO Pipettor, qPCR plates are automatically sealed and introduced to the qPRD thermal cycler.

Parallel production capacity allows for qPCR reations to be assembled while qPCR cycling tasks are executing. Automated qPCR Systems are available for any size project from 1 to 100 qPCR thermal cyclers

  • SOLO Pipettor in single or multi channel configurations
  • Tube Racks for almost any incoming sample tube
  • PlateCrane microplate robot
  • qPCR Thermal cycler(s)
  • Barcode Reader
  • Input and Output for qPCR Plate Storage
  • Easy to use and Flexible Softlinx Software

Automating your qPCR System Integration of your qPCR real-time system allows for significant walk-away time and continuous operation of your thermal cycler. Customers want to feed their PCR plates to their PCR detection system as soon as the PCR plates are prepared. This could mean starting an automated PCR process with as little as 1 PCR plate and continuously add PCR plates as they become available. Our integration software allows you to easily add PCR plates to an automated run without interruption.

Barcode driven protocols for real-time PCR detection systems are easy to setup. If several users want to run specific protocols for certain conditions related to the input samples. When this information is tied to the plate barcode, our system can easily allow them to control the real time PCR process.

Examples of Barcode Driven qPCR Protocols:

  • Real Time PCR Plates Barcoded with Suffix “A1” Run PCR protocol (1)
  • Real Time PCR Plates Barcoded with Suffix “A2” Run PCR protocol (2)

Scalability has never been easier. Are you automating multiple real-time qPCR devices? Hudson Robotics’ modular approach to automating qPCR easily accommodates two or more qPCR machines integrated with one PlateCrane, giving you unmatched value in your Automated Real-Time PCR process.

System access is hugely important for labs that have changing priorities. Some folks want to run qPCR samples manually, while others want to run several qPCR plates overnight. The Hudson approach to automated qPCR systems allows for both manual and fully automated operation of your qPCR system.

Advanced applications for the Roche LightCycler, ABI 7900 and Bio-Rad CFX systems are easily accommodated when integrating with Hudson Robotics. A popular integration is to push qPCR data to your LIMS. For some clients it is important to feed qPCR output data to their LIMS for analysis and storage as it becomes available. Hudson’s SoftLinx allows for data transfer to meet any protocol. Worklist generation as qPCR samples of interest are identified, SoftLinx can work with your LIMS to drive down straem qPCR activities, such as hit picking of targets for further processing or preparation for additional analysis.

Choosing Hudson Robotics for your first automated qPCR process or integrating our solutions into your automated facility brings unmatched value and scalability.

Feeding qPCR plates to any of the three major qPCR Systems below, using Hudson’s PlateCrane robot arm, is a rapid installation that can be completed in 1 day or less. Hudson offers proven performance for the following qPCR applications:

  • Automated Roche LightCycler Real-Time PCR System
  • Automated ABI 7900HT Fast Real-Time PCR System.
  • Automated Bio-Rad CFX Real-Time PCR Detection System
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