Hudson has built a complete gene assembly automation process, the key first step of the typical synthetic biology pipeline. We have systems that can be integrated with commercial automated oligo synthesizers, as well as subsequent de-protection and purification. In addition, we can pool oligos and carry them through the complete process to form functioning genes and complete genomes, such as viruses.
 We have automated solutions for each of the
steps of the Gene Assembly Sub-Pipeline. |
| Gene Assembly Sub-Pipeline |
- Click on the text or graphic in the pipeline to learn more about each step.
- Click the “Related Products” tab to learn more about the various options we provide to support Gene Assembly
- Contact us if you require a custom solution that isn’t currently on our list, or require more information about these products.
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Additional Information
Oligo Synthesis – This stage involves downloading desired oligo formulations and converting them into specific instructions for automated oligo synthesizers. Operator prompts ensure the availability of necessary materials.
Deprotection and Purification – The synthesized oligos are eluted from solid substrates and deprotected in an automated sequence of steps. This hands-free process includes rinsing synthesizer columns, adding deprotection reagents, performing the deprotection reaction, and subsequent plate handling.
Normalization – Through the utilization of absorbance microplate readers and adaptable instrument instructions, our system achieves normalized DNA concentrations in every well, ensuring consistency across samples.
Pooling – SoftLinx laboratory automation software guides the pipettor to pool the appropriate oligos as per the downloaded sequence instructions, facilitating the final assembly process to achieve the desired full gene sequence.
Gene Assembly – This concluding step involves a combination of PCR heating and cooling steps, orchestrated by SoftLinx-directed pipetting of requisite reagents. This culminates in the creation of a fully assembled gene.
Our approach empowers researchers with a robust, efficient, and customizable platform for gene assembly, leveraging cutting-edge technology and automation at every stage of the process
 - PCR and Gene Assembly Workstation
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