The Drug Discovery Process ELISA stands for enzyme-linked immunosorbent assay. It is an evaluation technique for immobilizing an antigen to a solid surface to determine peptide, protein, antibody, or hormone concentrations. The procedure uses an automated ELISA microplate reader in combination with a series of microplates—a plate with 96 indentations, or "wells"—that act as tiny [...]
ELISA stands for Enzyme-Linked ImmunoSorbent Assay. There are several types of ELISAs, including the sandwich ELISA, indirect ELISA, direct ELISA, and automated ELISA, all of which are used to detect antibodies. So, why is an ELISA sandwich assay associated with a popular lunch menu item? It is called a sandwich because it uses two antibodies, [...]
Automation in microbiology offers very distinct benefits: first, laboratory robotics and automation provide accuracy and efficiency, which is a benefit when many samples must be analyzed. However, there has also been a lack of staffing within the past several years. As of 2018, it was estimated that 17.4 percent of laboratory staff was expected to [...]
ELISA, which stands for Enzyme-Linked ImmunoSorbent Assay, is a type of immunological assay. Antigens from a sample are placed onto a surface, followed by an antibody in its most basic form. The antibody then must link to an enzyme, removing unbound antibodies. Finally, the enzyme’s substrate is added right before the detection phase. ELISA has [...]
There are 3 different types of ELISA that are widely used: direct ELISA, indirect ELISA, and ELISA sandwich assay. ELISA tests have been used to detect and diagnose HIV, Covid-19, food allergies, and many other conditions. You’ll learn how ELISA tests work, how different types of ELISA are used, and why scientists would choose one [...]
An ELISA (Enzyme-Linked ImmunoSorbent Assay) is a method used to determine protein concentrations in samples. Developed in 1971, it offered an alternative to radioimmunoassays, thus avoiding dangerous radioactive compounds. Used initially to evaluate immunoglobulins in serum, it quickly grew into a technique in identifying microbes, parasites, infections, and finally concentrations of hormones and proteins. It [...]
ELISA stands for enzyme-linked immunosorbent assay. It is an immunological assay that is most often used to detect and quantify proteins, antibodies/antigens, viruses, bacteria, and glycoproteins in life science and pharmaceutical laboratories. An ELISA can diagnose many diseases like COVID-19 and allow scientists to examine new drugs to verify their structure and look for potential [...]
A map of an ELISA plate is essentially a template of the 96 wells found on a standard ELISA plate. With 96 wells, each containing an individual sample, it is critical to track exactly what has been placed in each well. An ELISA plate map helps to do exactly that. An ELISA plate map is [...]
ELISA is based on the interaction of antibodies and antigens where the antibodies attach to specific proteins or nucleic acids. With measurable reporter molecules attached to the antibodies, it is possible to measure the amount of protein or nucleic acid present. Serial dilution ELISA is an important part of testing whenever examining an unknown peptide [...]
Can I Afford a Laboratory Automation System? Enzyme-linked immunosorbent assay (ELISA) is one of the most common testing methods for detecting antibodies to several proteins from the SARS-CoV-2 virus, the pathogen responsible for COVID-19. The spike protein (S) is found on the virus's surface and allows the virus to enter host cells. The nucleocapsid protein [...]
