ELISA Microplate Reader: Principle and Uses

The Drug Discovery Process ELISA stands for enzyme-linked immunosorbent assay. It is an evaluation technique for immobilizing an antigen to a solid surface to determine peptide, protein, antibody, or hormone concentrations. The procedure uses an automated ELISA microplate reader in combination with a series of microplates—a plate with 96 indentations, or "wells"—that act as tiny [...]

2022-08-16T15:16:59-04:00August 9th, 2022|Comments Off on ELISA Microplate Reader: Principle and Uses

What Is an ELISA Sandwich Assay?

ELISA stands for Enzyme-Linked ImmunoSorbent Assay. There are several types of ELISAs, including the sandwich ELISA, indirect ELISA, direct ELISA, and automated ELISA, all of which are used to detect antibodies. So, why is an ELISA sandwich assay associated with a popular lunch menu item? It is called a sandwich because it uses two antibodies, [...]

2022-05-18T17:57:35-04:00May 31st, 2022|Comments Off on What Is an ELISA Sandwich Assay?

Automation Opportunities in a Microbiology Lab

Automation in microbiology offers very distinct benefits: first, laboratory robotics and automation provide accuracy and efficiency, which is a benefit when many samples must be analyzed. However, there has also been a lack of staffing within the past several years. As of 2018, it was estimated that 17.4 percent of laboratory staff was expected to [...]

2022-04-26T17:29:49-04:00April 26th, 2022|Comments Off on Automation Opportunities in a Microbiology Lab

Automated ELISA and Its Applications

ELISA, which stands for Enzyme-Linked ImmunoSorbent Assay, is a type of immunological assay. Antigens from a sample are placed onto a surface, followed by an antibody in its most basic form. The antibody then must link to an enzyme, removing unbound antibodies. Finally, the enzyme’s substrate is added right before the detection phase. ELISA has [...]

2022-04-19T00:58:15-04:00April 19th, 2022|Comments Off on Automated ELISA and Its Applications

A Guide to Different Types of ELISA

There are 3 different types of ELISA that are widely used: direct ELISA, indirect ELISA, and ELISA sandwich assay. ELISA tests have been used to detect and diagnose HIV, Covid-19, food allergies, and many other conditions. You’ll learn how ELISA tests work, how different types of ELISA are used, and why scientists would choose one [...]

2022-05-25T15:23:10-04:00April 5th, 2022|Comments Off on A Guide to Different Types of ELISA

What a Fully-Automated ELISA Reader Can Do

An ELISA (Enzyme-Linked ImmunoSorbent Assay) is a method used to determine protein concentrations in samples. Developed in 1971, it offered an alternative to radioimmunoassays, thus avoiding dangerous radioactive compounds. Used initially to evaluate immunoglobulins in serum, it quickly grew into a technique in identifying microbes, parasites, infections, and finally concentrations of hormones and proteins. It [...]

2022-01-27T17:07:53-05:00January 25th, 2022|Comments Off on What a Fully-Automated ELISA Reader Can Do

Why Use A Fully Automated ELISA Reader?

ELISA stands for enzyme-linked immunosorbent assay. It is an immunological assay that is most often used to detect and quantify proteins, antibodies/antigens, viruses, bacteria, and glycoproteins in life science and pharmaceutical laboratories. An ELISA can diagnose many diseases like COVID-19 and allow scientists to examine new drugs to verify their structure and look for potential [...]

2021-10-18T16:41:48-04:00October 19th, 2021|Comments Off on Why Use A Fully Automated ELISA Reader?

How To Map An ELISA Plate

A map of an ELISA plate is essentially a template of the 96 wells found on a standard ELISA plate. With 96 wells, each containing an individual sample, it is critical to track exactly what has been placed in each well. An ELISA plate map helps to do exactly that. An ELISA plate map is [...]

2021-09-21T17:14:10-04:00September 21st, 2021|Comments Off on How To Map An ELISA Plate

Serial Dilution Method In ELISA: What & How

ELISA is based on the interaction of antibodies and antigens where the antibodies attach to specific proteins or nucleic acids. With measurable reporter molecules attached to the antibodies, it is possible to measure the amount of protein or nucleic acid present. Serial dilution ELISA is an important part of testing whenever examining an unknown peptide [...]

2021-08-10T12:27:46-04:00August 10th, 2021|Comments Off on Serial Dilution Method In ELISA: What & How

How ELISA Automation Can Speed Up COVID-19 Testing

Can I Afford a Laboratory Automation System? Enzyme-linked immunosorbent assay (ELISA) is one of the most common testing methods for detecting antibodies to several proteins from the SARS-CoV-2 virus, the pathogen responsible for COVID-19. The spike protein (S) is found on the virus's surface and allows the virus to enter host cells. The nucleocapsid protein [...]

2021-06-22T12:10:43-04:00June 22nd, 2021|Comments Off on How ELISA Automation Can Speed Up COVID-19 Testing
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