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Intellicyt iQue Screener

Hudson Robotics equipment

Intellicyt iQue Screener

Hudson has developed a SoftLinx plugin for the Intellicyt iQue Screener, making it easy to automate flow cytometry-based research efforts.   For example, integrating the iQue™ Screener with our PlateCrane EX microplate robot arm provides scientists with an automated sample acquisition system for screening cells and beads in solution.  This combination provides the user with a powerful system for screening large libraries with various cell and bead-based assays, on a bench top or in a hood.

This workcell offers small footprints for mobility and flexibility, and easy-to-use SoftLinx software for data tracking and scheduling.   SoftLinx communicated directly with Intellicyt’s ForeCyt™ software and automatically loads and executes stored protocols.  SoftLinx makes it easy to expand your system with the addition of other instrumentation, such as bar code scanners, liquid handlers, heating and cooling nests, and plate sealers.

The Flow Cytometry work cell supports a wide range of cell and bead-based assays.  A few general areas of research supported by this system include:

  • Small Molecule Cell-Based Drug Discovery
  • Antibody Engineering
  • Phenotypic Screening
  • in Vitro Toxicology Screening

See the Intellicyt website for more details on specific applications.

iQue Specifications – Click here for PlateCrane Specification

SYSTEM

 

Dimensions (W x D x H)

99 x 63 x 66 cm (39 x 25 x 26 inches)

Weight

82 kg (180 lb)

Sampling

Manual and with autosampler. Autosampler accommodates 96- and 384-well plates with plate recall auto settings. 1536-well is a planned future capability compatible with current hardware.

Minimum Volume Requirements

Zero dead volume, 1 µL sampled volume, 10 µL assay volume (96-well plates)

Screening Detection Method

Flow cytometry

Event Rate Detection

10,000/sec

Sensitivity Range

6+ decades

Gain and Voltage Settings

Automatic

Laser Excitation

Solid state lasers: blue (488 nm) and red (633 nm)

Multicolor Detection

4 simultaneous color channels using PMTs

Label-free Object Detection

2 light scatter channels for size granularity

Sensitivity

7 decade dynamic range using fixed optics

Minimum Plate Processing Speed

3 minutes for one 96-well plate, 12 minutes for one 384-well plate

SOFTWARE

Enterprise Data Software

Standard

Software Features

Standard: normalization, dynamic linked gating, interactive heat maps, 1D and 2D plot settings, custom PDF data report, well scan, unified work space, custom metrics, automated cleaning, one data file per plate, templates, advanced screening software. File size per 96-well plate: 70-100 MB. File export: FCS, CSV and ForeCyt plate formats.

Computer Requirements

Windows 7, 64-bit

SAFETY

CE Compliance

Yes

 

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