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RapidPick™ Harvester™ Colony Picker

A unique, automated colony picker for stem cell clusters, microbial and fungal samples in microplates or omnitrays.

The RapidPick™ Harvester™ identifies and extracts sample plugs of stem cell clusters, microbial colonies, or fungal samples grown on agar or in semi-solid media. This robotics picking platform is uniquely easy-to-use, accurate and durable.

Description

Equipped with a digital camera, the RapidPick™ Harvester™ Colony Picker  allows the researcher to manually identify sample locations across the microplate or omnitray, or to run any of a variety of imaging programs to automatically select qualified colonies or samples for picking. The Harvester then stores the image and selection coordinates for later picking, or can be directed to pick the selected samples right then. The extracted sample plugs are then transferred to a media-filled growth plate for outgrowth and testing. The Harvester is perfect for stem cell research.

The Harvester is also able to read the output files of cell imagers, like Brooks Automation’s Celligo™ , then go directly to the sample locations identified in their output files to extract individual plugs and transfer them to selected wells in a media-filled growth plate.

The Harvester’s ease-of-use is extraordinary. Users can learn how to operate it with only minutes of training. They can view selected picking locations of sample plates if they wish, and, with a simple point-and-click, delete or add locations at will. This can be done either at the system, or at their office computer.

Options include automated media filling, seal application/removal and incubation.

The RapidPick Harvester Colony Picker can be used for selecting fungal samples, identifying and sampling cellular clusters and for taking sample plugs out of gels. It also enables users to perform ordinary pipetting tasks, such as reformatting plates and transferring liquids among plates.

RapidPick™ Harvester™ Colony Picker: Cellular Samples

The RapidPick Harvester Colony Picker can be joined with cellular imagers, such as Brooks Automation’s Celligo™ imaging cytometer, to pick samples from microplate wells that have been imaged by that instrument. It does this by using the barcode ID of the plate, captured by the imager, then interprets the imager instrument’s output file for that plate. The Harvester can then accurately transform the location coordinates of selected cells, from the imager’s file, directly into coordinates on the Harvester’s deck to provide precise, complete picking of the identified cells.

Plugs from Gels

The Harvester can also serve as a fully-automated, single-tip colony picker, which can extract pin-sample or full plugs of gel-based microbial colonies. The Harvester uses disposable pipette tips, so there is no delay for cleaning and sterilization. Yet, each sample is fully isolated.

Specifications

Plate Capacity: 4 to 6 positions; up to 150 plates with optional PlateCrane accessory.
Plate Format: SBS standard footprint, any well configuration, or 4-inch Petri dish
Construction
Pipettor/picker unit: Stainless main deck; powder-coated steel upper arm covers over machined aluminum structural components;
Enclosure: Anodized aluminum frame, with Lexan sliding access doors.
Spill Protection: All electronics protected by solid, non-penetrated cover to prevent any leakage onto electrical devices
Arm Mechanism: Horizontal Travel/Vertical Stroke: x=22.5″; y=9.3″; z=4.0″
Size
Pipettor/picker unit: 24.0″ x 29.5″ x 19.5″ (H x W x D)
Enclosure: 36″ x 60.0″ x 33.0″ (H x W x D)
Weight: 50lbs; ~120lbs. w/ enclosure; ~165lbs. w/ PlateCrane
Power Requirements: 120-240 VAC, 50-60Hz, 2Amps Max at 120
Computer Interface: RS-232 serial cable

 

  • Fully-automated sample selection and picking
  • Manual-override selection prior to picking
  • Multiple image collection and analysis methods
  • Compatible with third-party cell imaging instruments
  • Includes email notification and remote operation
  • Archives images over time, for growth tracking
  • Harvests samples in both agar and liquid media
  • Uses sterile, barrier pipette tips to prevent cross-contamination
  • Offers full automation capability with plate-handling by Hudson’s PlateCrane
  • Includes the full pipetting features of a single-tip robotic pipettor
  • Plate reformatting is a standard feature
  • Easy-to-use operator screens
  • Intuitive menus and graphical interfaces

Applications

Fungal Samples

Colony Picking Fungal, Yeast, Stem Cell and Bacterial

Microbiome

Protein Binding

Synthetic Biology

Accessories

HEPA Filtration: Positive or negative pressure

UV Sterilization

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