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THE LAB AUTOMATION EXPERTS

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FilterPress for Nucleic Acid and Protein Extractions and Purifications

A superior solution to vacuum nests and centrifuges for filter-based extractions and purifications.

The positive pressure FilterPress for Extractions and Purifications is an affordable, flexible improvement over vacuum nests and centrifugation. Designed to handle ANSI SLAS-formatted filter plates and columns, FilterPress can triple the pressure differential available from vacuum nests. It is fully automatable and ready to be integrated with robotic liquid-handlers, robot arms, and reagent dispensers.

Description

In some instances, FilterPress for Nucleic Acid and Protein Extractions and Purifications can mitigate or even eliminate the need for centrifugation by exerting high pressure and flow on your filter columns. This lowers the cost and complexity of your process and makes automated loading far easier.

A digital pressure regulator assures constant pressure for optimal flow control and extraction for flow rate sensitive steps.

FilterPress for Extractions and Purifications is:

  • Highly flexible, easy-to-use software based on our popular flowchart-based SoftLinx™ automation system.
  • Can be run as a standalone module, semi-automated, with the optional Micro10x reagent dispenser, or integrated into the SOLO Robotic Liquid Handler.
  • Compatible with manual or robotic loading and operation as part of a PlateCrane integrated system.
  • One workstation can save and run multiple protocols for extracting DNA, RNA, and Proteins from Soil, Plants, Blood, etc.
  • Integrate easily with most liquid handlers and robot arms.
  • Provides easy access to upper and lower plate nests – upper for filter/extraction plates and lower for waste and collection.
  • Small footprint fits easily on benchtop.

Specifications

Handles ANSI/SLAS footprint filter plates or column arrays.
96-,48-,24-position, or any other ANSI/SLAS microplate format.
Provides more than 3 times the pressure differential and flow rate of vacuum nests with digital pressure/flow control.
Available with an optional heated air source (20° – 100 °C).
FilterPress Only 12” W x 16.5” D x 18.5” H
FilterPress with Micro10x™ 20.5” W x 16.5” D x 20” H
Filtration Pressure   Programmable, from 0 to half the air supply pressure
Filter Column Dimensions ANSI SLAS-standard footprint, 1” to 3.5” height
Collection Plate Dimensions ANSI SLAS-standard footprint, 0.4” to 2” height
Operating temperature Ambient to 100°C (with Heater Option)
Computer interface RS232
Power input 115V / 220V AC, 50/60 Hz
Air Supply 60 to 120psi; 10 SCFM @ 100psi max. flow

Applications

FilterPress has been designed to run commercial kits that extract DNA, RNA, and proteins from a variety of sources, including:

  • Soil
  • Plants
  • Bacteria
  • Blood
  • Urine
  • Saliva
  • and more…

The most evident application of FilterPress in the life sciences field is the isolation and purification of nucleic acids and proteins from various sources.

It can be employed in a wide range of protocols.  Here are a few examples:

DNA Extraction
DNA Purification
RNA Extraction
RNA Purification
Solid Phase Extraction (SPE)
Oligo Purification
Protein Expression & Purification
Plasmid Mini-Prep

Accessories

FilterPress can be used as a standalone instrument to carry out a wide variety of filtrations and separations.  However, a number of additional components can be added to partially or fully automate your protocols.

  1. Micro10x™ Dispenser– When attached to the FilterPress, this device can quickly add wash and elution buffers to the entire plate or any number of individual rows.
  2. 4 or 10-way valves – This Micro10x add-on allows the user to change the solvent at different stages of the protocol.
  3. SOLO™ Automated Pipettor – The FilterPress was designed to dock with our SOLO pipettor.  In this configuration, the SOLO can transfer liquids from various sources to and from the upper and lower nests of the FilterPress
  4. PlateCrane EX™or PlateCrane SciClops™ Robot Arms – Addition of a PlateCrane robot arm, with corresponding microplate stacks, will greatly increase the throughput of your protocol.
  5. Automated Magnetic Nest – Many protocols involving the extraction of DNA, RNA, and proteins involve filter-based separation techniques and magnetic bead-based techniques.  Replacing one of the SOLO nests with our automated Magnetic Bead nest will allow the user to mix and match separation techniques.  The software-controlled nest will raise the plate to allow proper liquid handling and lower the plate to allow the magnetic field to form pellets so the SOLO can remove the supernatant liquid.

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