Synthetic Biology Workstation
|Part of our new series of instruments designed to support the|
All of these functions are automated and integrated into a single, easy to use system.
• Pipetting/Hit Picking
• DNA Normalization
• Plating Colonies
• Gene Assembly
• Serial Dilutions
• Plasmid Prep
You provide the competent cells and the DNA Constructs, we’ll do the rest. The software tracks everything from start to finish. DNA is normalized and transformations carried out. Agar is spread on Omni trays and colonies are plated to your specifications.
Hudson has succeeded in automating the gene assembly process, the key first step of a typical synthetic biology pipeline. We have gene assembly solutions that can be integrated with commercial automated oligo synthesizers, as well as subsequent de-protection and purification. In addition, we can pool oligos and carry them through the complete process to form functioning genes and complete genomes, such as viruses.
DNA and RNA Normalization
Utilizing the simplicity of Hudson’s SOLO, and SoftLinx Software, users perform accurate DNA normalization of any plate of DNA samples. Using the SOLO to perform all the solvent addition, mixing and sample transfer operations, the user selects a DNA quantification file, and starts the system.
Obtaining pure DNA plasmids from bacteria are frequently carried out by lysing the cells and separating the plasmids through filter columns. Hudson offers a fully automated solution to this application. Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study.
Colony plating is a key process in various applications that require the replication and distribution of viable cell cultures. It can also be used to determine if a transformation procedure was successful in introducing an observable property upon a culture, such as the common process of introducing ampicillin resistance.