Hudson’s RapidPick SP is a single-pin version of Hudson’s widely-acclaimed RapidPick MP multi-pin colony-picker and re-arrayer. Like the RapidPick Complete and RapidPick Lite, the RapidPick SP has all the features needed by users in today’s molecular biology environment. The RapidPick SP has been designed to meet the needs of labs that need automated colony-picking and accurate data-handling, but whose throughput needs can be met by its medium-throughput productivity.
Its key features include:
Compact, lab bench size
Expandable to robotic arm loading
The RapidPick SP has all the colony-picking and colony-handling features of our other RapidPick models, and is designed for medium-throughput, typically 250 colonies per hour. The average inoculation rates of the SP are over 99%. The major competitors use batch picking so there is a delay in placing colonies into the destination plate. The delay may weaken the E. coli or other bacterial colonies or cause death. This microbial colony picker’s small footprint saves lab space. The RapidPick SP fits easily on most lab benchtops, and is ideal for use in microbiome research picking inside anaerobic chambers.
The imaging system, picking pin and sterilization system on the RapidPick-SP is the same as the imaging system, picking pin and sterilization system on the standard RapidPick. The differences are the plate loading and the number of pins used to pick samples.
The wash and sterilization system has been used on the picking system for over 10 years. Multiple customers have tested the sterilization of the system with PCR and there has never been an issue with contamination after the heater.
The following video shows the single-pin picker (RapidPick SP) in action
There is no need to change the pins for picking and reformatting applications. The RapidPick SP makes the change in software and uses the same pins. Larger diameter pins are available if desired. The standard pins transfer ~50nl of liquid. The system can be set up for multiple transfers from the same source if needed.
Using the RapidPick SP, users can manually load a colony plate and a media-filled target plate, then start the automated picking through an easy operator interface on the instrument’s control computer. Hudson’s RapidPick designed to pick from petri dishes, Nunc™ Omnitray ™ or any SBS plates, including: 1, 6, 8, 24, 48 and 96-well plates. They will inoculate SBS 1, 24, 96, 384, 1536 deepwell and standard height microplates. The SP includes barcode scanners to identify barcodes on each of the source colony plate and the target growth plate, if the user’s plates are barcoded. Then, the transfer data is saved using these barcodes to identify the resulting picked colonies. If the user’s plates are not barcoded, the data will be saved in sequential order. The system is fully compatible with Hudson’s PlateCrane EX robotic arm and SoftLinx scheduling software, already installed on the RapidPick-SP is ready to go for integration of the plate handling arm and stacks. Advanced integration with other instruments as part of a complete molecular biology workcell is easily done. Software Plug-Ins are available to communicate with all Hudson Robotics instruments and over 200 instruments from other manufactures such as LiCONiC incubators, media dispensers, robotic arms and more.
The RapidPick’s easy to use software enables users to create unique colony morphology sets to automate the colony picking process
Select the visual parameters for automatically identifying colonies such as: Radius, Amplitude, Elongation, Conformity, Separation, Colony Color
Select the Source Plate format to be used
Select the Target Plate format
Select the Type of operation (colony-picking, re-arraying, plate replication, etc.)
Select picking segments on segmented colony plates
Hudson Robotics can help researchers that are studying the microbiota (gut microbiome) in anaerobic chambers with its small form factor, automated tools for bacterial colony-picking, sample preparation, DNA extraction, plasmid preparation and DNA normalization. Research on Gastrointestinal disorders, Infectious diseases, metabolic disorders, immunology, oncology and CNS are all possible using Hudson technologies.
Hudson Robotics’ unique capabilities and product mix have enabled us to lead the entire world in the automation of the complete Synthetic Biology Pipeline: from automated oligo synthesis and gene assembly through transformation, colony picking and plasmid preparation.
Combined with our previous work with protein expression and purification systems, Hudson now offers a solution to automating any process encountered in a typical molecular biology laboratory.
Hudson has been a key player in the colony picking arena for many years. We offer several versions of our RapidPick platform, including the recently introduced single-pin picker. Easy-to-use, but sophisticated software interacts with the picker and the incorporated camera to identify and select appropriate colonies.
Easy-to-use, easy to set up, easy to maintain, easy to grow as applications change.
The software is easy to use and robust for colony picking
Re-arraying option for plate replication and cherry picking is included at no charge
Optional modules include: Halo picking, GFP picking, Colony counting, LED light panel in any color
Software is intuitive and requires no programming
Easily set maximum colonies to pick from segment or plate
Easily set control wells anywhere in the destination plate, 1, 2 or 3 wells or whole rows or columns. Controls are 100% user-defined
Includes basic service kit for minor issues like pin replacements
48 hour response time for field service in most areas around the world
Includes barcode scanners for source and destination plates at no charge and all data tracking.
The RapidPick SP is very economical with its low price and low cost maintenance.
The picking area is fully-enclosed for a clean and safe work environment.
The RapidPick SP can be expanded anytime to include automated media dispensing, shaking incubation, plate sealing and/or greater plate capacity.
Holds 1 source plate and 1 destination plate at a time
Uses a LED light box with the intensity of the lights controlled in software.
LED lights can be changed to different colors if desired.
Camera takes an image of the entire plate in a single image. No image cropping or building is required.
The user creates parameter files for identifying good colonies. There is no limit to the number of parameter files that can be created. Good colonies are determined by size, shape, contrast to background and closeness to other colonies.
The user has the option of manually editing the plate to be picked if desired. Colonies can be selected or deselected with the click of the mouse.
Operation of the picker is very fast and easy; scan image, analyze image, edit image and start picking.
Typical picking speed is 250 wells inoculated per hour
Can pick through the agar and hit the bottom of the plate with no damage to the pin, the linear slide or the plate.
The inoculation pin can be set to “wiggle or dither” in the destination well from 1-10 shakes, resulting in inoculation efficiency of 98%+.
Wash station is a vacuum wash to remove loose debris on the pin. After leaving the wash station the pin travels through a brush to remove anything remaining
Pins is sterilized with direct heat. The tungsten pin heats through and all sample on the pin is destroyed. The tungsten construction allows the pin to cool before being used for picking again.
The picker can support NUNC Omnitrays and Petri dishes for picking
The picker supports most SBS format shallow and deep well plates for the destination or in use with plate replication; 24 wells, 96 and 384 formats.