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RapidPick MP: 20 Pin Colony Picker

Hudson Robotics equipment

RapidPick MP: 20 Pin Colony Picker

RapidPick MP Colony PickerThe RapidPick MP 20 pin colony picker software is the fastest, most accurate automated colony picking system in the world.  The average inoculation rates of the MP are over 99%. The major competitors use batch picking so there is a delay in placing colonies into the destination plate. The delay may weaken E. coli  or other bacterial colonies or cause death.

This microbial colony picker’s small footprint saves lab space and easily fits into anaerobic chambers and biosafety hoods. The typical colony picking rate is around 2,400 colonies per hour. Software Plug-Ins are available to communicate with all Hudson Robotics instruments and over 200 instruments from other manufactures such as LiCONiC incubators, media dispensers, robotic arms and more. The open physical layout allows for easy integration with other instruments and cleaning. Hudson Robotics can provide chambers to showcase your workcell.

The RapidPick MP is intended for researchers who need the accuracy and high outgrowth rates, but whose workload doesn’t require the added automation available in the other versions of the RapidPick.

Using the RapidPick MP, users can manually load a colony plate and a media-filled target plate, then start the automated picking through an easy operator interface on the instrument’s control computer.  Hudson’s RapidPick  designed to pick from  petri dishes,  Nunc™ Omnitray ™  or any SBS plates, including: 1, 6, 8, 24, 48 and 96-well plates.  They will inoculate SBS 1, 24, 96, 384, 1536 deepwell and standard height microplates.  The MP includes barcode scanners to identify barcodes on each of the source colony plate and the target growth plate, if the user’s plates are barcoded.  Then, the transfer data is saved using these barcodes to identify the resulting picked colonies.  If the user’s plates are not barcoded, the data will be saved in sequential order. With the optional 2nd destination plate the user can create both an assay plate and an archive plate. Optional fluorescence detection and zone of exclusion bacterial colony detection is available. 

The RapidPick’s easy to use software enables users to create unique colony morphology sets to automate the colony picking process

  • Select the visual parameters for automatically identifying colonies such as: Radius, Amplitude, Elongation, Conformity, Separation, Colony Color 
  • Select the Source Plate format to be used
  • Select the Target Plate format
  • Select the Type of operation (colony-picking, re-arraying, plate replication, etc.)
  • Select picking segments on segmented colony plates
  • Define picking parameters and limits    

The Hudson Robotics RapidPick family of colony Pickers, Liquid Handlers, Microplate Handling systems and Laboratory Automation software are used in a wide range of applications in Synthetic Biology and fields such as Microbiome Research:
  • Clone Management
  • Gene Assembly
  • DNA transformation into Bacteria
  • Plasmid Prep
  • DNA/RNA/Protein extraction and purification
  • Next-Gen Sequencing (NGS)
  • Gene Construction
  • DNA and RNA normalization
  • Many More…
For the full list of Applications please click here.
Next-Gen Sequencing (NGS)

Next-Gen Sequencing (NGS)

Hudson Robotics automates the Next Generation Sequencing (NGS) pipeline, for high-quality processing of ready-for-next-gen sample: DNA Normalization, Magnetic Beads (Ampure) cleanup, DNA library construction and indexing, PCR Prep and PCR.


DNA Extraction

DNA Extraction

DNA/RNA/Protein Extraction – Isolate pure DNA from the cell.  There are a wide variety of protocols available for isolating pure DNA from all sorts of material.  But they all involve the same four basic steps: lysis, adsorption, washing and elution.  The details vary, depending on the source material and the concentration of DNA expected.

Microbiome Research

Microbiome Research

Hudson Robotics can help researchers that are studying the microbiota (gut microbiome) in anaerobic chambers with its small form factor, automated tools for bacterial colony-picking, sample preparation, DNA extraction, plasmid preparation and DNA normalization. Research on Gastrointestinal disorders, Infectious diseases, metabolic disorders, immunology, oncology and CNS are all possible using Hudson technologies.

Gene Construction

Gene Construction

The final step in the gene assembly sub-pipeline involves a combination of PCR cycles with pipetting of the appropriate reagents to create a completed gene construct.  

Oligo Pooling

Oligo Pooling

Hudson offers an automated solution for pooling of oligos.  Following the downloaded sequence instructions,  SoftLinx directs the system’s pipettor to pool the appropriate oligos to enable the final assembly process to achieve the full gene sequence desired.

Oligo Purification

Oligo Purification

Hudson offers a variety of solutions for purification of oligos. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer imperdiet tristique nunc, eu luctus nisi tempus eget. Cras tincidunt vestibulum sodales. Praesent lacinia luctus vestibulum. Phasellus condimentum eros eget leo gravida accumsan sit amet vitae dolor. Aenean sed nunc et sapien pellentesque aliquet. Praesent id orci risus. Duis pretium neque nisi, vel dignissim metus lacinia egestas. Nunc vel ante varius, varius quam et, consectetur nunc. Nunc sit amet elit quis nibh eleifend tristique id sit amet diam. Nulla a convallis purus. Ut laoreet tincidunt leo, lobortis eleifend est ornare vitae. In pulvinar eros nec lectus elementum, ac facilisis orci dictum. Nulla nisl quam, consectetur lobortis aliquam id, tristique a enim. Proin quis sollicitudin nulla. Ut varius turpis a placerat interdum.

Oligo Deprotection

Oligo Deprotection

Hudson offers two fully automated solution to deprotection of oligos.  The most efficient solution involves controlling the deprotection features of an automated oligo synthesizer.  Alternatively, we support various deprotection protocols with our liquid handling instruments.

Oligo Synthesis

Oligo Synthesis

Hudson has built a powerful new plugin for SoftLinx that allows the user to take maximum advantage of their oligo synthesizer, making it part of a larger automated gene assembly pipeline.  

Gene Assembly

Gene Assembly

Hudson has succeeded in automating the gene assembly process, the key first step of a typical synthetic biology pipeline.  We have gene assembly solutions that can be integrated with commercial automated oligo synthesizers, as well as subsequent de-protection and purification.  In addition, we can pool oligos and carry them through the complete process to form functioning genes and complete genomes, such as viruses.

Synthetic Biology

Synthetic Biology

Hudson Robotics’ unique capabilities and product mix have enabled us to lead the entire world in the automation of the complete Synthetic Biology Pipeline:  from automated oligo synthesis and gene assembly through transformation, colony picking and plasmid preparation.

Combined with our previous work with protein expression and purification systems, Hudson now offers a solution to automating any process encountered in a typical molecular biology laboratory.  

Plasmid Preparation

Plasmid Preparation

Obtaining pure DNA plasmids from bacteria are frequently carried out by lysing the cells and separating the plasmids through filter columns.  Hudson offers a fully automated solution to this application. Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study.

Fungal, Yeast, Stem Cell, Bacterial Colony Picking

Fungal, Yeast, Stem Cell, Bacterial Colony Picking

Hudson has been a key player in the colony picking arena for many years.  We offer several versions of our RapidPick platform, including the recently introduced single-pin picker.  Easy-to-use, but sophisticated software interacts with the picker and the incorporated camera to identify and select appropriate colonies.

DNA Transformation into Bacteria

DNA Transformation into Bacteria

Transformation, in which DNA is incorporated into a bacterial cell, is a key step in many biological and pharmaceutical applications.  Hudson has developed a number of automated systems for transforming cells by the heat shock approach.

Protein Expression & Purification

Protein Expression & Purification

Hudson offers a wide range of instrumentation to support the entire range of protocols commonly encountered when preparing peptides and proteins.  From liquid handlers to support PCR through vast, multi-component systems to support an entire research effort.

Features

  • Easy to use, easy to set up, easy to maintain, easy to grow as applications change
    • The software is easy to use and robust for colony picking
    • Re-arraying option for plate replication and cherry picking is included at no charge
      • Optional modules include: Halo picking, GFP picking, Colony counting, LED light panel in any color
    • All methods are ready to go and ready to use, no programming is required
    • Easily set maximum colonies to pick from segment or plate
    • Easily set controls wells anywhere in the destination plate, 1,2 or 3 wells or whole rows or columns.  Controls are 100% user defined
    • Lid removal and lid management is included at no charge.
    • Includes basic service kit for minor issues like pin replacements
    • 48 hour response time for field service in most areas around the world (*ask about your location)
    • Includes barcode scanners for source and destination plates at no charge and all data tracking.
    • Includes email notification of all errors, run completions and whatever information you need from the system
  • The RapidPick MP is very economical with its new low price and low cost maintenance program.  We’ve included more than ever at a more affordable price.
  • The new enclosure provides a clean and safe work environment
    • Options include: free standing table, HEPA filtration, UV sterilization
  • The RapidPick MP  is everything you want today and nothing you don’t.  If you need to add something tomorrow, it’s no problem with the RapidPick.  The system can be expanded anytime to include automated  media dispensing, shaking incubation, plate sealing and/or greater plate capacity.
    • On-board capacity for picking over 1700 colonies per run into 96 DW blocks
      • Options include: expansion up to 10,000 colony capacity, on-board shaking incubation, Automated media dispensing, on-board plate sealing

RapidPick Multi-Pin Colony Picking Systems consist of the CP-7200 colony-picking instrument, the PlateCrane EX (except MP model) with up to 10 stacks, and software development by Hudson Robotics to control the system.  The systems have the following specifications:

  • Software for colony picking, cherry picking, re-arraying and plate replication is standard.
  • Supports NUNC Omnitrays and Petri dishes as source plates for colony-picking.
  • Supports most SBS format shallow and deep well plates for the destination or in use with plate replication; 24 wells, 96, 384 and 1536 formats.
  • Comes standard with 2 barcode readers, 1 for the source plates and 1 for the destination plates.
  • Holds 1 source plate and 1 destination plate at a time, with option for 2 destination plates.
  • Has input capacity of 72 deep-well culture growth plates.
  • Includes barcode tracking of both colony and culture plate/well
  • Uses 20 tungsten pins on spring loaded linear slides attached to a rotating turret for picking.
  • Uses LED light box with the intensity of the lights controlled in software.
  • LED lights can be changed to different colors if desired.
  • Camera takes an image of the entire plate in a single image.  No image cropping or building is required.
  • User creates parameter files for identifying good colonies.  There is no limit to the number of parameter files that can be created.  Good colonies are determined by size, shape, contrast to background and closeness to other colonies. 
  • User can manually edit the plate to be picked if desired.  Colonies can be selected or deselected with the click of the mouse.
  • 4 pins are actuated at the same time, picking pin, inoculation pin, wash station, sterilization.  This action takes under 1 second.  After the pins are released, the rotor moves by 1 position.
  • Typical picking speed is 1 pick every 1-1.5 seconds; ~40 a minute, ~2400+ per hour.
  • Picking pin can pick through agar and hits bottom of plate with no damage to pin, linear slide or plate.
  • Inoculation pin can be set to “wiggle or dither” in destination well from 1-10 shakes.  This improves the inoculation efficiency to 98%+.
  • Wash station is a vacuum wash to remove loose debris on the pin.  After leaving wash station, pin travels through a brush to remove anything remaining.
  • Pins are sterilized with direct heat.  The pins enter a heater coil or furnace at 600F for under 1 second.  The tungsten pins heats through and all samples on the pins are destroyed.  Tungsten allows pin to cool before being used for picking. 

Optional Components

  • Media dispenser for fresh growth media in every plate.
  • Integrated gas-permeable seal applicator to insure freedom from contamination.
  • Available fully-integrated operation with products like Liconic’s STX 40 robotic incubator which provides complete automation of the entire culture outgrowth process.
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