Hudson Robotics has developed a unique, automated picker for
stem cell clusters, microbial and fungal samples in microplates or omnitrays.
The RapidPick Harvester™ identifies and extracts sample plugs of stem cell clusters, microbial colonies, or fungal samples grown on agar or in semi-solid media. It is uniquely easy-to-use, accurate and durable.
Equipped with an optional digital camera, the Harvester allows the researcher to manually identify sample locations across the microplate or omnitray, or to run any of a variety of imaging programs to automatically select qualified colonies or samples for picking. The Harvester then stores the image and selection coordinates for later picking, or can be directed to pick the selected samples right then. The extracted sample plugs are then transferred to a media-filled growth plate for outgrowth and testing.
The Harvester is also able to read the output files of cell imagers, like Brooks Automation’s Celligo™ , then go directly to the sample locations identified in their output files to extract individual plugs and transfer them to selected wells in a media-filled growth plate.
The Harvester’s ease-of-use is extraordinary. Users can learn how to operate it with only minutes of training. They can view selected picking locations of sample plates if they wish, and, with a simple point-and-click, delete or add locations at will. This can be done either at the system, or at their office computer.
Options include automated media filling, seal application/removal and incubation.
The RapidPick Harvester™ can be used for selecting fungal samples, identifying and sampling cellular clusters and for taking sample plugs out of gels. It also enables users to perform ordinary pipetting tasks, such as reformatting plates and transferring liquids among plates.
Equipped with its own high-resolution digital camera, the Harvester captures images of petri dishes or omnitrays containing fungal samples. These images are presented to the user to select where a sample should be harvested and transferred to a media-filled microplate well.
Users can, in the comfort of their own office, review these images and select locations with a point-and-click selection utility. The Harvester will automatically retrieve a fungal sample from each selected location and transfer it to a microplate for isolated outgrowth. If the system is using barcoded omnitrays and employs Hudson’s PlateCrane robot to load and unload the samples trays, these images can be queued for later review and plug selection. Then, they can be re-positioned by the robot back onto the Harvester’s deck for automated plug retrieval into the destination microplate(s).
The video below shows this version of the Harvester in action:
The RapidPick Harvester can be joined with cellular imagers, such as Brooks Automation’s Celligo™ imaging cytometer, to pick samples from microplate wells that have been imaged by that instrument. It does this by using the barcode ID of the plate, captured by the imager, then interprets the imager instrument’s output file for that plate. The Harvester can then accurately transform the location coordinates of selected cells, from the imager’s file, directly into coordinates on the Harvester’s deck to provide precise, complete picking of the identified cells.
Plugs from Gels
The Harvester can also serve as a fully-automated, single-tip colony picker, which can extract pin-sample or full plugs of gel-based microbial colonies. The Harvester uses disposable pipette tips, so there is no delay for cleaning and sterilization. Yet, each sample is fully isolated.
Hudson Robotics’ unique capabilities and product mix have enabled us to lead the entire world in the automation of the complete Synthetic Biology Pipeline: from automated oligo synthesis and gene assembly through transformation, colony picking and plasmid preparation.
Combined with our previous work with protein expression and purification systems, Hudson now offers a solution to automating any process encountered in a typical molecular biology laboratory.
Hudson has been a key player in the colony picking arena for many years. We offer several versions of our RapidPick platform, including the recently introduced single-pin picker. Easy-to-use, but sophisticated software interacts with the picker and the incorporated camera to identify and select appropriate colonies.
Protein Expression & Purification
Hudson offers a wide range of instrumentation to support the entire range of protocols commonly encountered when preparing peptides and proteins. From liquid handlers to support PCR through vast, multi-component systems to support an entire research effort.
The RapidPick HarvesterTM provides all the advanced features that researchers need to make harvesting fungal and microbial samples intuitive and easy:
- Fully-automated sample selection and picking
- Manual-override selection prior to picking
- Multiple image collection and analysis methods
- Compatible with third-party cell imaging instruments
- Includes email notification and remote operation
- Archives images over time, for growth tracking
- Harvests samples in both agar and liquid media
- Uses sterile, barrier pipette tips to prevent cross-contamination
- Offers full automation capability with plate-handling by Hudson’s PlateCrane
- Includes the full pipetting features of a single-tip robotic pipettor
- Plate reformatting is a standard feature
- Easy-to-use operator screens
- Intuitive menus and graphical interfaces
Plate Capacity: 4 to 6 positions; up to 150 plates with optional PlateCrane accessory.
Plate Format: SBS standard footprint, any well configuration, or 4-inch Petri dish
Pipettor/picker unit: Stainless main deck; powder-coated steel upper arm covers over machined aluminum structural components;
Enclosure: Anodized aluminum frame, with Lexan sliding access doors.
Spill Protection : All electronics protected by solid, non-penetrated cover to prevent any leakage onto electrical devices
Arm Mechanism: Horizontal Travel/Vertical Stroke: x=22.5″; y=9.3″; z=4.0″
Pipettor/picker unit: 24.0″ x 29.5″ x 19.5″ (H x W x D)
Enclosure: 36″ x 60.0″ x 33.0″ (H x W x D)
Weight : 50lbs; ~120lbs. w/ enclosure; ~165lbs. w/ PlateCrane
Power Requirements: 120-240 VAC, 50-60Hz, 2Amps Max at 120
Computer Interface: RS-232 serial cable
|This video shows fungal colonies being selected from a Petri dish with a RapidPick Harvester|
|This video shows a fungal colony picking protocol using the RapidPick Harvester|
|This video demonstrates Calibration, Imaging, Selection and Collection of samples using a RapidPick Harvester from a Petri dish.|