Synthetic Biology Workstation
Hudson Robotics’ unique capabilities and product mix have enabled us to lead the entire world in the automation of the complete Synthetic Biology Pipeline: from automated oligo synthesis and gene assembly through transformation, colony picking and plasmid preparation.
Combined with our previous experience with protein expression and purification systems, Hudson can now offer solutions for automating any process encountered in a typical molecular biology laboratory.
Learn more about our automated solutions for each of the following steps of the Synthetic Biology Pipeline.
|Synthetic Biology Pipeline|
Hudson has developed a plugin for the Biometra TRobot thermocycler. This integration has been incorporated into a number of workstations that require a PCR step as part of the supported application pipeline.
SOLO Liquid Handler
The Hudson SOLO™ liquid handling robot is an automated pipettor that can do anything expected of a hand-held pipettor perfectly, at about half the cost of other laboratory robotics platforms, without human error. The Solo works well below 1ul (1-3ul CV=1%; 0.25ul CV=4%). The Solo will fit into most biological safety cabinets and fume hoods. The SOLO can be loaded and operated manually, but is also compatible with robot loading systems from most manufacturers, including our PlateCrane EX.
RapidPick Complete Colony Picker
Hudson’s RapidPick™ Colony Picking Systems are the only fully automated high-throughput colony picking workcells. They deliver performance and precision unparalleled in the Life Science industry. In addition to allowing researchers to automate the selection and growth of cells grown on a colony plate, they also retain a record and image of each specific colony and which culture plate/well it inoculated.
RapidPick Lite Colony Picker
The RapidPick ‘Lite’ offers all the capacity, throughput and picking/re-arraying features of the RapidPick Complete, but without the liquid dispenser or microplate sealer. The RapidPick Lite averages 2,400 colonies per hour. The RapidPick Lite includes the RapidPick MP, a PlateCrane Robotic Arm and 5 plate stacks.
OB-1 Robotic Lab Assistant
A collaborative effort between Hudson Robotics and Obotics, this workcell supports a wide variety of sample preparation functions, including SPE/SLE processing, hydrolysis and derivativization, with all of the requisite microplate and liquid handling features required to automate the entire process.
RapidPick MP: 20 Pin Colony Picker
The RapidPick MP is the fastest, most accurate automated multi-pin colony picking system in the world with over 99% inoculation rates and typical speeds of picking over 2,400 bacterial colonies per hour. The small footprint saves lab space, allows for easy cleaning and fits into microbiome chambers and biosafety hoods. The RapiPick MP is at the heart of the Complete and Lite systems which include robotic plate loading, media filling as well as other features, such as plate sealing in the RapidPick Complete Workcell.
The RapidPick MP is intended for researchers who need the accuracy and high outgrowth rates of the RapidPick colony-picker, but whose workload doesn’t require the added automation available in the Complete or Lite systems.
Hudson’s RapidPick SP is a single-pin version of Hudson’s widely acclaimed RapidPick multi-pin colony-picker and re-arrayer. Like the RapidPick and RapidPick Lite, the RapidPick SP has all the features needed by users in today’s molecular biology environment.
Hudson Robotics has developed a unique, automated picker for microbial and fungal samples in microplates or omnitrays. The RapidPick Harvester™ identifies and extracts sample plugs of stem cell clusters, microbial colonies, or fungal samples grown on agar or in semi-solid media.
inheco ODTC 96
Hudson has developed a plugin for the inheco ODTC 96 thermocycler. This represents another integration that has been incorporated into workstations that require a PCR step as part of the supported application pipeline.
Roche LightCycler Integration
Hudson has developed a plugin for the Roche LightCycler high-throughput PCR instrument. Integration with the PlateCrane will allow you to take full advantage of the high level of stand-alone performance of this instrument.
- Wikipedia – Synthetic Biology
- Wikipedia – Genomics
- The Synthetic Biology Project at the Wilson Center
- The Synthetic Biology Center at MIT
- Synthetic Genomics
- Genetic Transformation Systems in Fungi, Volume 2 – Includes a chapter (16) entitled “Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals ,” written by Hudson Robotics and the USDA
“Systems and Methods for Automated Proteomics Research,”US 20070184546 A1 – Our US patent on the first integrated workcell to support the assembly and expression of modified genes
- Design and Construction of a First-Generation High-Throughput Integrated Robotic Molecular Biology Platform for Bioenergy Applications
- High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell
- Robot-controlled System Speeds “Designer” Yeasts for Making Ethanol
Transformation and Colony Plating
|This video shows automated bacterial transformation and colony plating using the Hudson SOLO Pipettor.|
|In the following video, DNA Normalization is carried out on the SOLO pipettor|
|In the following video, the SOLO plates 12 colonies at a time directly onto a Petri Dish. This is part of a system developed for the USDA.|
|This video shows the RapidPick ACIS in action which colonies to be picked are stored in a Liconic incubator.|
|In this video, the RapidPick is being used to pool several dye samples|
|Colonies can also be picked with the SOLO pipettor. This video shows fungal colonies being selected from a Petri dish with a single-channel SOLO|
|This modified RapidPick system was designed to inoculate 2 different destination plates with each picked colony|