Obtaining pure DNA plasmids from bacteria are frequently carried out by lysing the cells and separating the plasmids through filter columns. Hudson offers a fully Plasmid Prep Workcell to this application. Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study.
|Synthetic Biology Pipeline|
Plasmid Preparation is a key part of the following Applications Pipelines that we support:
SOLO Liquid Handler
The Hudson SOLO™ liquid handling robot is an automated pipettor that can do anything expected of a hand-held pipettor perfectly, at about half the cost of other laboratory robotics platforms, without human error. The Solo works well below 1ul (1-3ul CV=1%; 0.25ul CV=4%). The Solo will fit into most biological safety cabinets and fume hoods. The SOLO can be loaded and operated manually, but is also compatible with robot loading systems from most manufacturers, including our PlateCrane EX.
RapidPick MP: 20 Pin Colony Picker
The RapidPick MP is the fastest, most accurate automated multi-pin colony picking system in the world with over 99% inoculation rates and typical speeds of picking over 2,400 bacterial colonies per hour. The small footprint saves lab space, allows for easy cleaning and fits into microbiome chambers and biosafety hoods. The RapiPick MP is at the heart of the Complete and Lite systems which include robotic plate loading, media filling as well as other features, such as plate sealing in the RapidPick Complete Workcell.
The RapidPick MP is intended for researchers who need the accuracy and high outgrowth rates of the RapidPick colony-picker, but whose workload doesn’t require the added automation available in the Complete or Lite systems.