Hudson has succeeded in automating the complete gene assembly process, the key first step of the typical synthetic biology pipeline. We have systems that can be integrated with commercial automated oligo synthesizers, as well as subsequent de-protection and purification. In addition, we can pool oligos and carry them through the complete process to form functioning genes and complete genomes, such as viruses.
|We have automated solutions for each of the |
steps of the Gene Assembly Sub-Pipeline.
|Gene Assembly Sub-Pipeline|
- Click on the text or graphic in the pipeline to learn more about each step.
- Click the “Related Products” tab to learn more about the various options we provide to support Gene Assembly
- Contact us if you require a custom solution that isn’t currently on our list, or require more information about these products.
Oligo Synthesis – Downloads desired oligo formulations from a local file or via internet. Converts the file information into specific instructions for an automated oligo synthesizer, as well as operator prompts to insure that the required materials are present.
Deprotection and Purification – Elutes the synthesized oligos from their solid substrates, then deprotects them in a seamless, fully automated series of steps. These steps include a rinse under vacuum of the synthesizer columns, addition of deprotection reagents, sealing the collection plate (if necessary), performing the deprotectiion reaction, then unsealing the plate. All this is done in a hands-free automated process.
Normalization – Utilizing an absorbance microplate reader and SoftLinx’s ability to adapt its instrument instructions to the results of the reader, the system’s SOLO automated pipettor performs a series of sample transfers and diluitions to achieve a normalized DNA concentration in every plate well.
Pooling – Following the downloaded sequence instructions provided at the beginning of the process, SoftLinx laboratory automation software directs the system’s pipettor to pool the appropriate oligos to enable the final assembly process to achieve the full gene sequence desired.
Gene Assembly – The final step in the gene production process involves a combination of PCR heating and cooling steps with SoftLinx-directed pipetting of the appropriate reagents to create a completed gene assembly.
- PCR and Gene Assembly Workstation