Hudson has succeeded in automating the complete gene assembly process, the key first step of the typical synthetic biology pipeline. We have systems that can be integrated with commercial automated oligo synthesizers, as well as subsequent de-protection and purification. In addition, we can pool oligos and carry them through the complete process to form functioning genes and complete genomes, such as viruses.
|Synthetic Biology Pipeline|
|We have automated solutions for each of the |
steps of the Gene Assembly Sub-Pipeline.
|Gene Assembly Sub-Pipeline|
Oligo Synthesis – Downloads desired oligo formulations from a local file or via internet. Converts the file information into specific instructions for an automated oligo synthesizer, as well as operator prompts to insure that the required materials are present.
Deprotection and Purification – Elutes the synthesized oligos from their solid substrates, then deprotects them in a seamless, fully automated series of steps. These steps include a rinse under vacuum of the synthesizer columns, addition of deprotection reagents, sealing the collection plate (if necessary), performing the deprotectiion reaction, then unsealing the plate. All this is done in a hands-free automated process.
Normalization – Utilizing an absorbance plate reader and SoftLinx’s ability to adapt its instrument instructions to the results of the reader, the system’s SOLO pipettor performs a series of sample transfers and diluitions to achieve a normalized DNA concentration in every plate well.
Pooling – Following the downloaded sequence instructions provided at the beginning of the process, SoftLinx directs the system’s pipettor to pool the appropriate oligos to enable the final assembly process to achieve the full gene sequence desired.
Gene Assembly – The final step in the gene production process involves a combination of PCR heating and cooling steps with SoftLinx-directed pipetting of the appropriate reagents to create a completed gene assembly.
Hudson has developed a plugin for the Biometra TRobot thermocycler. This integration has been incorporated into a number of workstations that require a PCR step as part of the supported application pipeline.
SOLO Liquid Handler
The Hudson SOLO™ liquid handling robot is an automated pipettor that can do anything expected of a hand-held pipettor perfectly, at about half the cost of other laboratory robotics platforms, without human error. The Solo works well below 1ul (1-3ul CV=1%; 0.25ul CV=4%). The Solo will fit into most biological safety cabinets and fume hoods. The SOLO can be loaded and operated manually, but is also compatible with robot loading systems from most manufacturers, including our PlateCrane EX.
inheco ODTC 96
Hudson has developed a plugin for the inheco ODTC 96 thermocycler. This represents another integration that has been incorporated into workstations that require a PCR step as part of the supported application pipeline.
Roche LightCycler Integration
Hudson has developed a plugin for the Roche LightCycler high-throughput PCR instrument. Integration with the PlateCrane will allow you to take full advantage of the high level of stand-alone performance of this instrument.
- Genetic Transformation Systems in Fungi, Volume 2 – Includes a chapter (16) entitled “Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals ,” written by Hudson Robotics and the USDA
“Systems and Methods for Automated Proteomics Research,”US 20070184546 A1 – Our US patent on the first integrated workcell to support the assembly and expression of modified genes
|Gene assembly using the SOLO integrated with the Biometra TRobot.|