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DNA Extraction

Hudson Robotics equipment

DNA Extraction

DNA/RNA/Protein Isolation – Isolate pure DNA from the cell.  There are a wide variety of protocols available for isolating pure DNA from all sorts of material.  But they all involve the same four basic steps: lysis, adsorption, washing and elution.  The details vary, depending on the source material and the concentration of DNA expected.

Hudson provides components that support each of the commonly encountered variations of these steps.  In particular, the recent introduction of our FilterPress  means we can support all possible methods of adsorbing nucleic acids from the lysis mixture: vacuum filtration, pressure-based filtration, magnetic bead adsorption, and centrifugation.  The latter method is frequently the most difficult to automate, but is frequently required due to the lack of force produced by the vacuum-based methods.

The pressure-based FilterPress makes it possible to eliminate the centrifuge step in many situations.  The pressure-based method also greatly improves the ability to run extractions on a partially filled microplate without the need to cover the unused wells.  Vacuum nests are notorious for their inability to maintain an equal flow rate when some wells are empty.

Below is a simple DNA extraction system with each of the Hudson components labeled.  A simple system like this can be used to carry out plasmid preparation and a wide variety of other DNA/RNA or protein isolations.  

In this example, the samples are in Matrix tubes sitting in a shaker nest.  Lysing reagents are drawn from the deep-well reservoir on the SOLO and mixed with the samples.  The mixture is then adsorbed on the filter plates, sitting on the upper nest of the FilterPress.  At this stage, there wouldn’t be a collection plate in the lower nest so that a series of wash buffers can be added by the Micro10x dispenser.  A 4-way valve controls which buffer is added, and the lines are automatically emptied and primed as necessary.

In the final step, a collection plate is placed into the lower nest of the FilterPress and the elution buffer is added to the filter plate.  This separates the pure DNA from the filter and releases it into the collection plate.

This system can be fully automated and additional features can be included by adding the right equipment.  For example, the following image shows a more complex system that includes a PlateCrane for moving microplates, a UV reader for measuring DNA yields, and a thermocycler for amplifying the final product.  The thermocycler can also be used in protocols that require amplification as part of the isolation procedure, such as the 16S RNA isolation protocol, commonly used in microbiome research.

SOLO Liquid Handler

SOLO Liquid Handler

The Hudson SOLO™ liquid handling robot is an automated pipettor that can do anything expected of a hand-held pipettor perfectly, at about half the cost of other laboratory robotics platforms, without human error. The Solo works well below 1ul (1-3ul CV=1%; 0.25ul CV=4%). The Solo will fit into most biological safety cabinets and fume hoods. The SOLO can be loaded and operated manually, but is also compatible with robot loading systems from most manufacturers, including our PlateCrane EX.

Automated Extraction Systems (DNA, RNA and Proteins)

Automated Extraction Systems (DNA, RNA and Proteins)

Hudson Robotics has introduced another member of our expanding catalog of powerful systems designed to support genetic research efforts.  Our automated extraction system greatly simplifies the process of isolating pure nucleic acids and/or proteins from a wide variety of natural sources.

Extraction Tools - FilterPress™

Extraction Tools - FilterPress™

Hudson Robotics introduces the FilterPress, a powerful alternative to vacuum nests, magnetic beads and centrifuges for automated DNA Purification, Plasmid Preps, and Solid Phase Extraction of any kind. It does the same job as a vacuum manifold, but it’s capable of higher pressure than vacuum, useful for difficult samples. The FilterPress is fully articulated, so you can walk away. And if you’re used to vacuum, you’ll probably find it much less messy.

External Links

Here’s a short demo of some of the functionality of the Extraction System:

And here’s a video of the FilterPress in action:

Finally, this video shows a dry run of the Plasmid Preparation protocol on our system:

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