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Fungal, Yeast, Stem Cell, Bacterial Colony Picking

Hudson Robotics equipment

Fungal, Yeast, Stem Cell, Bacterial Colony Picking

Hudson Robotics is a colony picking leader.  The RapidPick family of instruments includes the RapidPick SP, the recently introduced single-pin colony picker, and the well-known 20-pin RapidPick colony picker.  RapidPick technology uses a camera to capture a single image of the entire petri dish, omni-tray or segmented colony plate, and then uses advanced image analysis to allow users to create selection profiles, maybe to select the maximum number of bacterial colonies, or discriminate among them for favored traits. The RapidPick Harvester is commonly used in stem cell research and it is used in the study of yeast and fungus. All of the Hudson’s bacterial colony picker technologies can fit in anaerobic chambers so they are ideal for microbiome research.

Product Highlights:  Press the “Related Products” tab to see some of the ways in which these instruments have been automated to support specific applications and levels of throughput.


Synthetic Biology Pipeline


Colony Picking is a key part of the following Applications Pipelines that we support:

SOLO Liquid Handler

SOLO Liquid Handler

The Hudson SOLO™ liquid handling robot is an automated pipettor that can do anything expected of a hand-held pipettor perfectly, at about half the cost of other laboratory robotics platforms, without human error. The Solo works well below 1ul (1-3ul CV=1%; 0.25ul CV=4%). The Solo will fit into most biological safety cabinets and fume hoods. The SOLO can be loaded and operated manually, but is also compatible with robot loading systems from most manufacturers, including our PlateCrane EX.

RapidPick Complete Colony Picker

RapidPick Complete Colony Picker

Hudson’s RapidPick™  Colony Picking Systems are the only fully automated high-throughput colony picking workcells. They deliver performance and precision unparalleled in the Life Science industry.  In addition to allowing researchers to automate the selection and growth of cells grown on a colony plate, they also retain a record and image of each specific colony and which culture plate/well it inoculated.

RapidPick Lite Colony Picker

RapidPick Lite Colony Picker

The RapidPick ‘Lite’ offers all the capacity, throughput and picking/re-arraying features of the RapidPick Complete, but without  the liquid dispenser or  microplate sealer. The RapidPick Lite averages 2,400 colonies per hour. The RapidPick Lite includes the RapidPick MP, a PlateCrane Robotic Arm and 5 plate stacks.

RapidPick MP: 20 Pin Colony Picker

RapidPick MP: 20 Pin Colony Picker

The RapidPick MP is the fastest, most accurate automated multi-pin colony picking system in the world with over 99% inoculation rates and typical speeds of picking over 2,400 bacterial colonies per hour.  The small footprint saves lab space, allows for easy cleaning and fits into microbiome chambers and biosafety hoods. The RapiPick MP is at the heart of the Complete and Lite systems which include robotic plate loading, media filling as well as other features, such as plate sealing in the RapidPick Complete Workcell. 

The RapidPick MP is intended for researchers who need the accuracy and high outgrowth rates of the RapidPick colony-picker, but whose workload doesn’t require the added automation available in the Complete or Lite systems.    

RapidPick SP

RapidPick SP

Hudson’s RapidPick SP is a single-pin version of Hudson’s widely acclaimed RapidPick multi-pin colony-picker and re-arrayer. Like the RapidPick and RapidPick Lite, the RapidPick SP has all the features needed by users in today’s molecular biology environment.

RapidPick Harvester

RapidPick Harvester

Hudson Robotics has developed a unique, automated picker for microbial and fungal samples in microplates or omnitrays.  The RapidPick Harvester™ identifies and extracts sample plugs of stem cell clusters, microbial colonies, or fungal samples grown on agar or in semi-solid media.

External

 

Pin-Based Picking – Colony Picking with the RapidPick Complete

This video shows the RapidPick in action which colonies to be picked are stored in a Liconic incubator.

 

In this video, the RapidPick is being used to pool several dye samples

 

This modified RapidPick system was designed to inoculate 2 different destination plates with each picked colony

The following video shows the single-pin picker (RapidPick SP) in action

Pipette-Based Picking – Fungal Picking on the RapidPick Harvester

Colonies can also be picked with the SOLO pipettor. This video shows fungal colonies being selected from a Petri dish with a single-channel SOLO

 

This video shows a fungal colony picking protocol using the RapidPick Harvester

 

This video demonstrates Calibration, Imaging, Selection and Collection of samples using a RapidPick Harvester from a Petri dish.

 

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