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DNA Normalization

Hudson Robotics equipment

DNA Normalization

Hudson’s DNA Normalization System and Built-in Wizard

Hudson’s DNA Normalization System is designed to take the tedium and uncertainty out of manual normalization pipetting steps, and insure accurate, documented normalized DNA samples.

Hudson’s DNA Normalization System consists of a SOLO Pipettor with an 8-channel pipette head, a 4-position landscape plate deck, the SOLO DNA Normalization Wizard and a laptop control computer for the operator GUI and to control the process.  Optional accessories include a shaker nest and a hand-held barcode scanner.

Hudson offers every user a choice of two DNA Normalization Methods:

1. Rapid Normalization (with varying final well volumes):  add diluent amounts to destination plate first, then add samples.

Method procedure:

  • Scan (or enter) sample DNA plate ID
  • Scan (or enter) normalized destination plate ID
  • Place rack of disposable tips on deck
  • Select saved protocol file (or create new one with desired settings)
  • Select data file for reader
  • System calculates diluent volume for each individual well in the destination plate
  • System adds calculated diluent amount to each destination plate well
  • System transfers constant volume of DNA sample from every well into the destination plate to achieve the desired (constant) concentration of DNA in the destination plate, using fresh tip for each well, mixing after each dispense, then discarding the tips

User Inputs:  (all can be saved to a protocol file, then the file can be called up instead of re-entering all the settings):

  1. Desired final DNA concentration
  2. Sample transfer volume (constant)
  3. Minimum final well volume
  4. Maximum final well volume
  5. Data file from absorbance reader

2. Constant Normalized Sample Volumes (with constant final well volumes): add varying diluents amounts to destinatuion plate first, then varying amounts of DNA sample to achieve a constant final well volume.

Method procedure:

  • Scan (or enter) sample DNA plate ID
  • Scan (or enter) normalized destination plate ID
  • Place rack of disposable tips on deck
  • Select saved protocol file (or create new one with desired settings)
  • Select data file for reader
  • System calculates diluent volume for each individual well in the destination plate
  • System adds calculated diluent amount to each destination plate well
  • System transfers calculated volume of DNA sample from every well in the DNA plate into the destination plate to achieve a uniform volume of normalized sample in each well of the destination plate, using fresh tip for each well, mixing after each dispense, then discarding the tips

User Inputs:  (all can be saved to a protocol file, then the file can be called up instead of re-entering all the settings):

  1. Desired final DNA concentration
  2. Desired final well volume
  3. Data file from absorbance reader

Additionally, the user will have saved the minimum and maximum  allowable pipetting volumes per well per transfer, based upon the pipettor head capacity and disposable tips being used.  Also, the user may select the option to:

  1. Ignore wells where the amount of initial DNA is too small or too dilute to allow the desired final concentration to be achieved
  2. Prompt the user to plate an intermediate plate on the deck to allow a dual dilution of samples where the initial concentration of DNAS is too high to achieve the desired concentration in only one dilution step.
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